Use of Pleural Fluid Interferon-gamma Enzyme-linked Immunospot Assay in the Diagnosis of Pleural Tuberculosis

Authors

  • Tika Adilistya Department of Clinical Pathology, Cipto Mangunkusumo Hospital, Jakarta, Indonesia
  • Dalima AW Astrawinata Department of Clinical Pathology, Cipto Mangunkusumo Hospital, Jakarta, Indonesia
  • Ujainah Z Nasir Department of Clinical Pathology, Cipto Mangunkusumo Hospital, Jakarta, Indonesia

Keywords:

interferon-γ release assay, ELISPOT, pleural tuberculosis

Abstract

Aim: to evaluate the diagnostic value of an interferon-gamma release assay (IGRA) with enzyme-linked immunospot (ELISPOT) method, T-SPOT.TB, in the diagnosis of pleural TB using pleural fluid mononuclear cells (PFMC). Methods: forty-eight subjects, presumed to have pleural TB with exudative pleural effusion by Light’s criteria, dominated by mononuclear cells, had their pleural fluid specimen tested with T-SPOT.TB, Mycobacterial Growth Indicator Tube (MGIT) culture, and adenosine deaminase (ADA) activity. Other causes of pleural effusion such as heart failure, renal failure, hepatic cirrhosis, and malignancy were excluded. Results: the sensitivity, specificity, positive predictive value, and negative predictive value of the IGRA ELISPOT assay using PFMC for the diagnosis of pleural TB were 100%, 88.89%, 97.5%, and 100%, respectively. Conclusion: IGRA with ELISPOT method performed on PFMC is useful for a rapid and reliable diagnosis of pleural TB in clinical practice, especially in area with high TB burden.

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Published

2016-09-19

How to Cite

Adilistya, T., Astrawinata, D. A., & Nasir, U. Z. (2016). Use of Pleural Fluid Interferon-gamma Enzyme-linked Immunospot Assay in the Diagnosis of Pleural Tuberculosis. Acta Medica Indonesiana, 48(1), 41. Retrieved from https://www.actamedindones.org/index.php/ijim/article/view/136

Issue

Vol. 48 No. 1 (2016): Acta Medica Indonesiana

Section

ORIGINAL ARTICLE

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