Induction of Rat Germ Cell Apoptosis by Testosterone Undecanoate and Depot Medroxyprogesterone Acetate and Correlation of Apoptotic Cells with Sperm Concentration

Authors

  • Syafruddin Ilyas Department of Biology, Faculty of Mathematics and Natural Sciences, University of North Sumatra. Indonesia
  • Silvia W Lestari Department of Medical Biology, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia
  • Nukman Moeloek Department of Medical Biology, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia
  • Asmarinah Asmarinah Department of Medical Biology, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia
  • Siregar C Siregar Department of Anatomy and Pathology, Faculty of Medicine, University of Indonesia, Jakarta, Indone

Abstract

Aim: to investigate the safety and effectiveness of dose TU+DMPA hormones in developing potential male contraceptives. Methods: suppression of rat sperm concentration through increased apoptotic germ cells by in vivo administration of a long-acting androgen composed of a combination of testosterone undecanoate (TU) and depot medroxyprogesterone acetate (DMPA) was performed. Adult Sprague-Dawley rats received 2.5 mg TU every 6 week and 1.25 mg DMPA every 12 week for 60 week, a regimen known to rapidly reduce testosterone production by the testes and produce azoospermia within 12 week. Sperm concentration data were log transformed before analysis. Results are expressed as the mean±SEM. ANOVA, followed by post hoc test was used to determine differences across time and phase. T test was employed to determine differences between two groups. Results: apoptosis revealed significant increase in apoptotic germ cells (80% when rats were administered with TU+DMPA. Apoptotic germ cells can be found in several spermatogonia (20%), spermatocytes (30%), and spermatids (50%). Conclusion: dose TU+DMPA hormones may be a safe and effective way to develop potential male contraceptives. Key words: TU, DMPA, sperm concentration, apoptosis.

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Published

2016-05-18

How to Cite

1.
Ilyas S, Lestari SW, Moeloek N, Asmarinah A, Siregar SC. Induction of Rat Germ Cell Apoptosis by Testosterone Undecanoate and Depot Medroxyprogesterone Acetate and Correlation of Apoptotic Cells with Sperm Concentration. Acta Med Indones [Internet]. 2016May18 [cited 2026Nov.7];45(1). Available from: https://www.actamedindones.org/index.php/ijim/article/view/118

Issue

Vol. 45 No. 1 (2013)

Section

ORIGINAL ARTICLE

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