Vitamin D Prevents Endothelial Damage Induced by Increased Neutrophil Extracellular Traps Formation in Patients with Systemic Lupus Erythematosus

Authors

  • Kusworini Handono Department of Clinical Pathology, Faculty of Medicine, Universitas Brawijaya - Saiful Anwar Hospital, Malang, Indonesia.
  • Yona One Sidarta Biomedical Sciences Master Program, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
  • Benny A Pradana Biomedical Sciences Master Program, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
  • Radhitio A Nugroho Biomedical Sciences Master Program, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
  • Ivan A Hartono Biomedical Sciences Master Program, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
  • Handono Kalim Department of Internal Medicine, Faculty of Medicine, Universitas Brawijaya - Saiful Anwar Hospital, Malang, Indonesia
  • Agustina T Endharti Department of Parasitology, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia

Abstract

Aim: to investigate the effects of Vitamin D calcitriol/1,25(OH)2D3 on NETosis in systemic lupus erythematosus (SLE) patients with hypovitamin D. Methods: neutrophlis of five SLE patients with hypovitamin D were divided into 4 groups, P0 (0 nM/control), P1 (1 nM), P2 (10 nM), and P3 (100 nM) as cultured samples. Phorbol Myristate Acetate (PMA) was used to stimulate NETs formation. The supernatant was separated and cocultured with HUVECs. Externalization of Neutrophil Elastase (NE) and Myeloperoxidase (MPO) during NETosis was measured by immunofluorescence and ELISA respectively. Early and late apoptosis of endothelial cell was measured by flowcytometry using cell death kit (Annexin V and PI antibody). Results: this study showed significant decrease in early apoptosis with 10 nM of 1,25(OH)2D3 compared to control group. Significance of NE externalization found in all treatment groups (p<0.05), while MPO absorbance in the same tendency but not statistically significant. Further analysis also found a moderate positive correlation between NE externalizations with early apoptosis. Conclusion: vitamin D 1,25(OH)2D3 could reduce endothelial damage by decreasing NETosis activity. This result may reveal the possibility of Vitamin D as supplementary therapy for SLE patients with hypovitamin D to prevent endothelial damage. Key words: NETs, NE, MPO, 1,25(OH)2D3, HUVECs.

Downloads

Published

2016-05-16

How to Cite

Handono, K., Sidarta, Y. O., Pradana, B. A., Nugroho, R. A., Hartono, I. A., Kalim, H., & Endharti, A. T. (2016). Vitamin D Prevents Endothelial Damage Induced by Increased Neutrophil Extracellular Traps Formation in Patients with Systemic Lupus Erythematosus. Acta Medica Indonesiana, 46(3). Retrieved from https://www.actamedindones.org/index.php/ijim/article/view/88

Issue

Vol. 46 No. 3 (2014)

Section

ORIGINAL ARTICLE

License

Copyright

The authors who publish this journal agree to the following requirements:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work. (See The Effect of Open Access)
Privacy Statement

The names and email addresses entered in this journal site will be used exclusively for the stated purposes of this journal and will not be made available for any other purpose or to any other party.